Interaction between Antagonist of Cannabinoid Receptor and Antagonist of Adrenergic Receptor on Anxiety in Male Rat

Authors

  • Abdolrahman Sarihi Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
  • Alireza Komaki Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
  • Fatemeh Abdollahzadeh Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
  • Iraj Salehi Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
  • Siamak Shahidi Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Abstract:

Introduction: Anxiety is among the most common and treatable mental disorders. Adrenergic and cannabinoid systems have an important role in the neurobiology of anxiety. The elevated plus-maze (EPM) has broadly been used to investigate anxiolytic and anxiogenic compounds. The present study investigated the effects of intraperitoneal (IP) injection of cannabinoid CB1 receptor antagonist (AM251) in the presence of alpha-1 adrenergic antagonist (Prazosin) on rat behavior in the EPM. Methods: In this study, the data were obtained from male Wistar rat, which weighing 200- 250 g. Animal behavior in EPM were videotaped and saved in computer for 10 min after IP injection of saline, AM251 (0.3 mg/kg), Prazosin (0.3 mg/kg) and AM251 + Prazosin, subsequently scored for conventional indices of anxiety. During the test period, the number of open and closed arms entries, the percentage of entries into the open arms of the EPM, and the spent time in open and closed arms were recorded. Diazepam was considered as a positive control drug with anxiolytic effect (0.3, 0.6, 1.2 mg/kg). Results: Diazepam increased the number of open arm entries and the percentage of spent time on the open arms. IP injection of AM251 before EPM trial decreased open arms exploration and open arm entry. Whereas, Prazosin increased open arms exploration and open arm entry. This study showed that both substances in simultaneous injection have conflicting effects on the responses of each of these two compounds in a single injection. Discussion: Injection of CB1 receptor antagonist may have an anxiogenic profile in rat, whereas adrenergic antagonist has an anxiolytic effect. Further investigations are essential for better understanding of anxiolytic and anxiogenic properties and neurobiological mechanisms of action and probable interactions of the two systems.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

interaction between antagonist of cannabinoid receptor and antagonist of adrenergic receptor on anxiety in male rat

introduction: anxiety is among the most common and treatable mental disorders. adrenergic and cannabinoid systems have an important role in the neurobiology of anxiety. the elevated plus-maze (epm) has broadly been used to investigate anxiolytic and anxiogenic compounds. the present study investigated the effects of intraperitoneal (ip) injection of cannabinoid cb1 receptor antagonist (am251) i...

full text

interaction between l-type calcium channels and antagonist of cannabinoid system on anxiety in male rat

conclusions ip injection of cb1 receptor antagonist might have an anxiogenic profile in rat, whereas calcium channel blocker attenuated the anxiogenic effect of am251. our results suggest that there is an interaction between functions of l-type ca2+ channels and cannabinoid system in anxiety. background the elevated plus-maze (epm) has been broadly used to investigate anxiolytic and anxiogenic ...

full text

Survey on the interaction effect of dopamine D2 receptor antagonist on morphine-induced polycystic ovary syndrome in rat

Background and Objective: Polycystic ovary syndrome (PCOS) is one of the most common endocrine and metabolic disorders in premenopausal women. Opioid drugs, including morphine are effective inducers of the PCOS. Hyperprolactinemia also increases the likelihood of this complication. The dopamine, the inhibitor of prolactin secretion, has receptors in the ovarian tissue. In this study, metoclopra...

full text

Repeated administration of cannabinoid receptor agonist and antagonist impairs short and long term plasticity of rat’s dentate gyrus in vivo

Introduction: The effects of cannabinoids (CBs) on synaptic plasticity of hippocampal dentate gyrus neurons have been shown in numerous studies. However, the effect of repeated exposure to cannabinoids on hippocampal function is not fully understood. In this study, using field potential recording, we investigated the effect of repeated administration of the nonselective CB receptor agonist WIN5...

full text

P 17: Electrophysiological Effects of Cannabinoid Receptor Antagonist AM251 on Harmaline Toxicity in Rat’s Cerebellar Vermis Slices

Introduction: The Cannabinoid receptors (CBR) densities are high within the cerebellum. Cannabinoid receptors manipulations have been reported to cause altering the cerebellar functions. harmaline have immune-modulatory effects in several studies. i.e., significant anti-inflammatory effect via the inhibition of prostaglandin E2 (PGE2) and tumor necrosis factor alpha (TNF-α). Endocannabino...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 5  issue 3

pages  218- 224

publication date 2014-07

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023